In order of appearance…
- First Member/Joy – Audrey Parkinson
- Second Member/Aranea – Hyrum Knighton
- Third Member/Nellie – Layleigh Stone
- Young Wilbur/Third Fairgoer – Brandon Watson
- Fern Arable – Reagan Paul
- Martha Arable – Isabelle Gailey
- John Arable – Dillon Krahenbuhl
- Avery Arable – Carter Lefler
- Wilbur – Nate Myers
- Edith Zuckerman – Eliza Wolff
- Homer Zuckerman – Carson Cannon
- Lurvy, the farmhand – Devn Draper
- Goose – Jayne Brinley
- Gander – Jack Peterson
- Goat/Judge – Avery Dow
- Kid/Judge – Morgyn Martin
- Sheep – Alder Nielsen
- Lamb – Emma Peart
- Mule/Judge – Eli Pickett
- Rooster/Henry Fussy* – Tray Anderson
- Hen* – Scarlet Washburn
- Templeton, the rat – Kamyla Pederson
- Charlotte, the spider – Emma Stewart
- Reporter* – Julia Mundy
- Photographer* – Rebecca Dome
- Uncle, a pig* – Owen Parker
- First Spectator* – Charlie Norwood
- Second Spectator* – Kate Weekes
- First Fairgoer* – Michaela Kessel
- Second Fairgoer* – Morgan Hansen
- Announcer* – James Moon
* indicates web puppeteer
All characters will also perform in ensemble roles throughout the show.